Composite

Part:BBa_K3883030:Design

Designed by: Liu Yuqi   Group: iGEM21_Jilin_China   (2021-10-20)


targeted knockdown the expression of ppc gene through CRISPRi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1208
    Illegal NheI site found at 4231
    Illegal NheI site found at 4254
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 49


Design Notes

targeted knockdown the expression of ppc gene through CRISPRi to downregulate TCA cycle


Source

targeted knockdown the expression of ppc gene through CRISPRi to downregulate TCA cycle

References